Chronic Treadmill Running Affects Adipose Tissue Metabolism in Spontaneously Hypertensive Rats

Ferreira A, Prímola-Gomes T, Menezes Z, Cruz J, Natali A. Chronic Treadmill Running Affects Adipose Tissue Metabolism in Spontaneously Hypertensive Rats. JEPonline 2010;13(5): 9-18. We investigated in adipocytes of hypertensive rats (SHR) the effects of chronic treadmill running on parameters influenced by insulin (anti-lipolytic effect, glucose uptake) and on LPL activity. Male SHR were randomly divided into two groups: chronic exercise (CEX-SHR, n=10) and sedentary control (SEDSHR; n=10). Lipolysis was measured under basal, isoproterenol (ISO, 0.1 μM) and insulin (4.3 nM) stimulated conditions. Glucose uptake was measured under basal and insulin (4.3 nM) stimulated conditions. Lipoprotein lipase (LPL) activity was also measured. Lipolytic activity and anti-lipolytic effect of insulin were higher in CEX (0.64 ± 0.06 mM) than in SED group (0.43 ± 0.09 mM). Insulin-stimulated glucose uptake increased in CEX compared to SED group (4.08 ± 0.18 nmol/3 min CEX vs. 3.06 ± 0.23 nmol/3 min SED). LPL activity decreased in CEX compared to SED group (0.22 ± 0.06 nmol [H]-fatty acid released/min, CEX vs. 0.36 ± 0.04 nmol [H]-fatty acids released/min, SED). In conclusion, chronic treadmill running increased SHR adipocyte lipolysis response to ISO stimulation and insulin-stimulated glucose uptake with decreased LPL activity at resting conditions.